Journal: bioRxiv

Article Title: Exploring the vomeronasal organ in an endangered antelope species

doi: 10.1101/2023.03.09.531847

Figure Lengend Snippet: All the lectins employed labelled the vomeronasal glands (VG) and the muco-microvillar complex, i.e. the superficial border of both vomeronasal epithelia. A, E . Labelling of the VNO at levels L3 and L8, respectively with the lectin SBA ( Glycine max ). In both cases the VG are strongly labelled. B. VVA ( Vicia villosa ) labelling at a central level L6 of the VNO shows a faint labelling of the VG. C, D. LEA ( Lycopersicon esculentum ) labels the vomeronasal epithelia, the NVNs, and the VG. LEA does not stain the caudal nasal nerve (asterisk in C). F, I, K, N. STA ( Solanum tuberosum ) lectin labelling at central levels (L4, L5) of the VNO stains the vomeronasal epithelia, vomeronasal nerves (NVN) and VG. G, H, J, L, M. UEA ( Ulex europaeus ) lectin stains vomeronasal epithelia, VG, but hardly stains NVNs. Vv, veins; directionality is indicated by the following: d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: A-C, E, I, J = 500 μm; D, F, G = 250 μm; H = 100 μm; K-N = 50 μm.

Article Snippet: STA (Vector-B1165), which is isolated from potato, Solanum tuberosum , binds oligomers of N-acetylglucosamine and some oligosaccharides containing N- acetylglucosamine and N-acetylmuramic acid ( ).

Techniques: Staining

Journal: Cell

Article Title: Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

doi: 10.1016/j.cell.2017.10.012

Figure Lengend Snippet: Identification of FMM-Constituent Lipids (A) Ion chromatogram of FMM lipid markers in DRM (left) and DSM (right) fractions, labeled with RT and m/z ratios. Lipid abundance represented in absorbance units (B) Fragmentation pattern of FMM lipid markers at negative (top) and positive (bottom) ESI by product ion scan (MS/MS). Common fragments with respective MW and tentative formulas are shown. (C) (Top) TLC detection of staphyloxanthin lipids in DRM and DSM fractions of WT and Δ crt mutant. Staphyloxanthin lipids are visualized as yellow-pigmented bands (arrowheads). (D) UV-visible spectroscopy of purified staphyloxanthin and DRM and DSM samples (WT and Δ crt mutant). Arrowheads indicate characteristic 463- and 490-nm staphyloxanthin peaks. (E) Fluorescein-labeled lectin binding assay to WT and Δ crt DRM samples. WGA, wheat germ agglutinin; STL, Solanum tuberosum lectin; RCA, Ricinus communis agglutinin; DBA, Dolichos biflorus agglutinin; UEA, Ulex europaeus agglutinin; ConA, concanavalin A. (F) Relative abundance of FMM lipid markers in WT and Δ crt mutant using ion chromatography. Data shown as mean ± SD for three biological replicates (n = 3). (G) Tentative molecular structure and fragmentation pattern (blue, negative ESI; red, positive ESI) of staphyloxanthin-related FMM lipid markers. See also and and and .

Article Snippet: Fluorescein Solanum tuberosum (potato) Lectin , Vector Laboratories , Cat#FL-1161.

Techniques: Labeling, Tandem Mass Spectroscopy, Mutagenesis, Spectroscopy, Purification, Binding Assay, Ion Chromatography

Journal: Cell

Article Title: Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

doi: 10.1016/j.cell.2017.10.012

Figure Lengend Snippet:

Article Snippet: Fluorescein Solanum tuberosum (potato) Lectin , Vector Laboratories , Cat#FL-1161.

Techniques: Recombinant, Plasmid Preparation, Electron Microscopy, Protease Inhibitor, Software, Western Blot, Microscopy, Mass Spectrometry, Size-exclusion Chromatography, Fluorescence, Tomography

Journal: Brain Pathology

Article Title: Common Impact of Chronic Kidney Disease and Brain Microhemorrhages on Cerebral Aβ Pathology in SHRSP

doi: 10.1111/bpa.12384

Figure Lengend Snippet: Aβ CNS deposition, microhemorrhages and tubulointerstitial renal damage in SHRSP. Dense parenchymal (A–A3 & C1), pericapillary (B, C (arrows) & C2–C4) Aβ deposits, small perivascular bleeds (D, E & F (arrows) & D1), infarcts (arrowheads in G) and IgG leakage throughout the small vessel walls (K–M) are part of the age‐dependent mixed cerebral pathologies in SHRSP. Iron and Aβ depositions are colocalized in the rat brain (A1–A3 & C1–C4). In the brains of these animals, recent microhemorrhages co‐occur with small ischemic lesions (arrowheads in E & F), thromboses (* in F) and with liquefaction ischemic lesions containing haem‐laden macrophages (arrowheads in G). Peritubular capillary erythrocyte accumulations (H, arrow), tubular protein cylinders (I, arrow) and hyperplastic arteriolosclerosis (J, arrows) indicate combined tubulointerstitial and vascular/hypertensive kidney damage in SHRSP. B, K, L, M – IHC, STL ‐ solanum tuberosum lectin‐fluorescein isothiocyanate (endothelial marker), zymo – in situ zymography showing matrix metalloproteinases 2,9 activity visualized with the use of gelatin conjugated with FITC (DQ gelatin, Invitrogen), IgG – immunoglobulin G, representing the IgG leakage, visualized with the use of an anti‐rat IgG secondary antibody Cy3 conjugated (Dianova, Hamburg, Germany, 1:500), DAPI ‐ 4′.6‐diamidino‐2‐phenylindole (nuclear staining), A–A3 – Prussian blue/CR staining, C, C2–C4 – Prussian blue/Thioflavine S staining, C1 ‐ Prussian blue/Thioflavine T staining, D–J – HE staining. A & A1 – corpus callosum, A2, A3, C1, C2 & D–G – cortex, B, C, C 3 & C4 – hippocampus, D1 ‐ basal ganglia, H & I – kidney medulla, J – kidney cortex. A1, B1, C3, C4 – magnifications of the respective subfigures.

Article Snippet: Five brain sections per animal adjacent to those with suspected CR‐positive Aβ deposits were stained with STL‐FITC (solanum tuberosum lectin‐fluorescein isothiocyanate, Axxora, Enzo Life Sciences GmBH, Loerrach, Germany, 1:500, endothelial marker), anti‐rodent Aβ (Covance, Dedham, MA, USA, 1:500, specific for rodent Aβ), and DAPI (4′.6‐diamidino‐2‐phenylindole, MoBiTec GmbH, Germany, 1:10 000, nuclear staining).

Techniques: Marker, In Situ, Zymography, Activity Assay, Staining